1x hot firepol evagreen qpcr mix plus (Solis BioDyne)

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Solis BioDyne 1x hot firepol evagreen qpcr mix plus
1x Hot Firepol Evagreen Qpcr Mix Plus, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firepol+evagreen+qpcr+mix+plus/HOT+FIREPol+EvaGreen+qPCR+Mix+Plus/10__1038_slash_s41598___026___73593___6-181-5-12
Average 99 stars, based on 1814 article reviews

1x hot firepol evagreen qpcr mix plus - by Bioz Stars, 2026-11

99/100 stars

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Article Title: Homologous recombination mutants cause differing lethality between h- and h+ Schizosaccharomyces pombe strains due to mat1 heterochromatin.
Article Snippet: .. GenTLE (from Yeast) High Recovery Kit (Takara, Kyoto, Japan). qPCR reactions (20 lL total volume) contained 25 ng of genomic DNA, 4 pmol of each primer (Table S3), and 19 HOT FIREPol EvaGreen qPCR Mix Plus (Solis BioDyne, Tartu, Estonia). .. Amplification was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with the following cycling conditions: 12 min at 95 °C; 40 cycles of 95 °C for 15 s, 60 °C for 20 s, and 72 °C for 20 s. Signal normalization was carried out using the act1 reference gene.

Real-time Polymerase Chain Reaction:

Article Title: QRFP43 modulates the activity of the hypothalamic-pituitary-thyroid axis in female sheep
Article Snippet: .. Real-time qPCR was performed using 5× FIREPol EvaGreen qPCR Mix Plus (no ROX; Solis BioDyne, Tartu, Estonia) following the protocol described by Szlis et al. (2018) . .. Relative gene expression was calculated using the comparative quantitation option implemented in Rotor Gene 6000 software 1.7 (Qiagen GmbH, Hilden, Germany), as well as the Relative Expression Software Tool and the PCR efficiency correction algorithm developed by Pfaffl (2002, 2004) , .

Article Title: QRFP43 modulates the activity of the hypothalamic-pituitary-thyroid axis in female sheep
Article Snippet: .. Scientific Reports | (2025) 15:1085 3| https://doi.org/10.1038/s41598-025-85693-w Real-time qPCR was performed using 5× FIREPol EvaGreen qPCR Mix Plus (no ROX; Solis BioDyne, Tartu, Estonia) following the protocol described by Szlis et al. (2018)29. .. Relative gene expression was calculated using the comparative quantitation option implemented in Rotor Gene 6000 software 1.7 (Qiagen GmbH, Hilden, Germany), as well as the Relative Expression Software Tool and the PCR efficiency correction algorithm developed by Pfaffl (2002, 2004)30,31.

Article Title: The role of QRFP43 in the secretory activity of the gonadotrophic axis in female sheep
Article Snippet: .. Real Time qPCR was performed using 5 × FIREPol EvaGreen qPCR Mix Plus (no ROX; Solis BioDyne, Tartu, Estonia) in a total volume of 15 μL containing 3 μL of Master Mix, 9 μL of RNase-free H 2 O, 2 × 0.5 μL of primers (0.5 mM), and 2 μL of cDNA template. .. Amplification was performed using a Rotor Gene 6000 thermocycler (Corbett Research, Mortlake, Australia) according to the protocol described by Przybył et al. .

Article Title: QRFP43 modulates somatotrophic axis activity in female sheep
Article Snippet: .. The primer sequences are shown in Table 1 (Przybył et al., 2021b) Real Time qPCR was conducted using 5× FIREPol EvaGreen qPCR Mix Plus (noROX; Solis BioDyne, Tartu, Estonia) in a total volume of 15 μl containing 3 μl Master Mix, 9 μl RNA-se free H2O, 2 × 0.5 μl primers (0.5 mM), and 2 μl cDNA template was used. .. Amplification was performed using a Rotor Gene 6000 thermocycler (Corbett Research, Mortlake, Australia) according to the following protocol: one cycle at 95 °C for 15 min (enzyme activation) followed by 35 cycles at 94 °C for 5 s (denaturation), 59 °C for 20 s (annealing), and 72 °C for 15 s (elongation) and one cycle at 72 °C for 7 min (product stabilisation).

Article Title: Tissue-Slice Organ-on-Chip Culture of Hypothalamic and Pituitary of Lambs─The Role of Phoenixin-20 as a Modulator of Gonadotrophic Axis.
Article Snippet: .. Real-time qPCR was performed using 5× FIREPol EvaGreen qPCR Mix Plus (no ROX; Solis BioDyne, Tartu, Estonia) in a total volume of 15 μL containing 3 μL of Master Mix, 9 μL of RNase-free H2O, 2 × 0.5 μL of primers (0.5 mM), and 2 μL of cDNA template. .. Amplification was performed using a Rotor Gene 6000 thermocycler (Corbett Research, Mortlake, Australia) according to the protocol described by Przybył et al. (2024).30 The experiment included glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin (ACTB), and peptidylprolyl isomerase C (PPIC).

Article Title: Tissue-Slice Organ-on-Chip Culture of Hypothalamic and Pituitary of LambsThe Role of Phoenixin-20 as a Modulator of Gonadotrophic Axis
Article Snippet: .. Real-time qPCR was performed using 5× FIREPol EvaGreen qPCR Mix Plus (no ROX; Solis BioDyne, Tartu, Estonia) in a total volume of 15 μL containing 3 μL of Master Mix, 9 μL of RNase-free H 2 O, 2 × 0.5 μL of primers (0.5 mM), and 2 μL of cDNA template. .. Amplification was performed using a Rotor Gene 6000 thermocycler (Corbett Research, Mortlake, Australia) according to the protocol described by Przybył et al. (2024).

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